Optimization and Production of SiderophoreProduction and optimization of siderophore from plant growth promoting rhizobacteriaAbstractThe aim of this study was to optimize the production of siderophores by bacterial strains isolated from rhizosphere soil. Chrome azurol sulphonate assay confirms siderophore production by all30 bacterial isolates. Maximum siderophore production was observed with strains S-6 and S-26 on standard succinic acid medium. Siderophore production was found to be influenced by different carbon, nitrogen and amino acid sources. Optimization of SM medium nutrient composition enhances siderophore production. The pot culture experiment clearly demonstrate the beneficial effect of strain S-6 and S-26 with significance increase in biometric parameters of soybean. Effect of rhizospheric bacterial isolates (S-6, S-26) on siderophore production was estimated by detecting the iron in soil as well as plant by using atomic absorption spectrophotometer. The iron concentration of soil was decreased after treatment from 38.32 ppm to 26.
